The PNL - BDNF - IRES 2 - EGFP plasmid was identified to be correct.
构建的慢病毒载体质粒PNL -BDNF-IRES2-EGFP 经SalI和 BamHI 双酶切鉴定正确,三质粒共转染293T细胞 后荧光激发可见大量绿色荧光.
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